标题： 通过侧细胞表面和基底细胞表面对管腔形态的控制 显示英文标题

标题： Control of lumen morphology by lateral and basal cell surfaces

摘要： 在发育过程中，由上皮组织包围的充满液体的腔体的形态来源于腔体压力、细胞皮层力学和细胞间粘附之间的相互作用。 在这里，我们利用MDCK囊肿的形状空间以及紧密连接扰动下其顶面的不稳定性，探讨这种相互作用的机械基础 [Mukenhirn等，Dev. Cell 59, 2886 (2024)]。 我们发现，除了已知的压力和顶面带张力的调节外，囊肿还会显著调节其侧向和基底张力以响应这些扰动。 我们开发了一个平均场三维顶点模型来模拟这些囊肿，该模型能够定量地再现实验中的形状不稳定性。 这表明观察到的侧向收缩性的增加是一种细胞反应，用以对抗不稳定性。 我们的工作因此展示了如何通过调节所有细胞表面的力学特性来共同控制腔体形态。 显示英文摘要

摘要： Across development, the morphology of fluid-filled lumina enclosed by epithelial tissues arises from an interplay of lumen pressure, mechanics of the cell cortex, and cell-cell adhesion. Here, we explore the mechanical basis for the control of this interplay using the shape space of MDCK cysts and the instability of their apical surfaces under tight junction perturbations [Mukenhirn et al., Dev. Cell 59, 2886 (2024)]. We discover that the cysts respond to these perturbations by significantly modulating their lateral and basal tensions, in addition to the known modulations of pressure and apical belt tension. We develop a mean-field three-dimensional vertex model of these cysts that reproduces the experimental shape instability quantitatively. This reveals that the observed increase of lateral contractility is a cellular response that counters the instability. Our work thus shows how regulation of the mechanics of all cell surfaces conspires to control lumen morphology.