标题： 红细胞中大分子动力学的中子谱学研究 显示英文标题

标题： Macromolecular Dynamics in Red Blood Cells Investigated Using Neutron Spectroscopy

摘要： 我们展示了对人红细胞内血红蛋白（Hb）动力学的中子散射测量。 在全球和内部Hb动力学方面，在皮秒到纳秒的时间尺度和{\AA }长度尺度上使用准弹性中子背散射光谱法进行了测量。 我们观察到了从全局Hb短时间自扩散到长时间自扩散的交叉现象。 当考虑到约0.23g H2O/g Hb的结合水分数时，短时间和长时间扩散系数在数量上与非带电硬球悬浮液的流体力学理论预测值一致。 与完全水合的Hb粉末相比，细胞中的水分更多，这促进了皮秒时间尺度上的内部蛋白质涨落。 发现红细胞中Hb在纳秒时间范围内的较慢内部动力学与完全水合蛋白质粉末、溶液和大肠杆菌细胞的结果相当。 显示英文摘要

摘要： We present neutron scattering measurements on the dynamics of hemoglobin (Hb) in human red blood cells in vivo. Global and internal Hb dynamics were measured in the ps to ns time- and {\AA} length-scale using quasielastic neutron backscattering spectroscopy. We observed the cross-over from global Hb short-time to long-time self-diffusion. Both short- and long-time diffusion coefficients agree quantitatively with predicted values from hydrodynamic theory of non-charged hard-sphere suspensions when a bound water fraction of around 0.23g H2O/ g Hb is taken into account. The higher amount of water in the cells facilitates internal protein fluctuations in the ps time-scale when compared to fully hydrated Hb powder. Slower internal dynamics of Hb in red blood cells in the ns time-range were found to be rather similar to results obtained with fully hydrated protein powders, solutions and E. coli cells.